anti fab Search Results


anti human fab  (Gator Bio Inc)
94
Gator Bio Inc anti human fab
Anti Human Fab, supplied by Gator Bio Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti igg hrp  (SouthernBiotech)
93
SouthernBiotech anti igg hrp
Anti Igg Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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biotinylated donkey anti rabbit  (Jackson Immuno)
93
Jackson Immuno biotinylated donkey anti rabbit
Biotinylated Donkey Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monovalent goat anti mouse fab fragments  (Jackson Immuno)
93
Jackson Immuno monovalent goat anti mouse fab fragments
Monovalent Goat Anti Mouse Fab Fragments, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse igg2b coating  (SouthernBiotech)
96
SouthernBiotech goat anti mouse igg2b coating
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Goat Anti Mouse Igg2b Coating, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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unconjugated fab 241 fragment goat anti rabbit igg  (Jackson Immuno)
96
Jackson Immuno unconjugated fab 241 fragment goat anti rabbit igg
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Unconjugated Fab 241 Fragment Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rabbit fab fragment antibody  (Jackson Immuno)
93
Jackson Immuno anti rabbit fab fragment antibody
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Anti Rabbit Fab Fragment Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human igg fc  (Bethyl)
94
Bethyl human igg fc
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Human Igg Fc, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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nipure fab fragment donkey antirabbit igg jackson immunoresearch  (Jackson Immuno)
94
Jackson Immuno nipure fab fragment donkey antirabbit igg jackson immunoresearch
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Nipure Fab Fragment Donkey Antirabbit Igg Jackson Immunoresearch, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cy3 conjugated affinipure f ab 2 fragment anti rabbit igg  (Jackson Immuno)
93
Jackson Immuno cy3 conjugated affinipure f ab 2 fragment anti rabbit igg
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Cy3 Conjugated Affinipure F Ab 2 Fragment Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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donkey jackson immunoresearch 711 167 003 goat  (Jackson Immuno)
93
Jackson Immuno donkey jackson immunoresearch 711 167 003 goat
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Donkey Jackson Immunoresearch 711 167 003 Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa 647 conjugated goat anti mouse fab  (Jackson Immuno)
93
Jackson Immuno alexa 647 conjugated goat anti mouse fab
Fig. 1. <t>IgG2b</t> I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.
Alexa 647 Conjugated Goat Anti Mouse Fab, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. IgG2b I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.

Journal: International immunology

Article Title: An expressed neo(r) cassette provides required functions of the 1gamma2b exon for class switching.

doi: 10.1093/intimm/10.11.1683

Figure Lengend Snippet: Fig. 1. IgG2b I exon and splice donor targeting of F1 and homozygous ES cells. (A) Construct. Approximately 290 bp of the I exon (SacI–HincII) along with 77 bp downstream of the splice donor site are deleted. Triangles represent loxP sites. The first 110 bp and the Iγ2b promoter are left intact to retain most of the transcription initiation sites (five out of seven retained). Restriction site abbreviations: S, SacI; H, HindIII; B, BamHI; H2, HincII. pIγ2b refers to the endogenous promoter for Iγ2b. (B) The targeting construct 6UTlN1X was transfected into ES cells and targeted cells were identified by Southern blot analysis using probe 1. The Cre recombinase gene was transiently transfected into ES cells homozygous for the PGK-neor gene to delete the neor gene.

Article Snippet: ELISA analysis Splenocyte culture supernatants from day 4.5 or 5 and sera were analyzed for the presence of different Ig isotypes by sandwich ELISA assay as previously described (13,28) using goat anti-mouse IgG2b coating (Southern Biotechnology Associates) and alkaline phosphatase-labeled anti-mouse IgG2b (R12-3) (PharMingen).

Techniques: Construct, Transfection, Southern Blot

Fig. 2. In vitro analysis of splenocytes from Iγ2b-targeted chimeric mice. (A) FACS analysis of IgG2b surface expression in mutant and control mice on day 4.5 of splenocytes cultured in RPMI/10% FCS medium containing LPS (20 µg/ml) with or without rIL-4 (20 ng/ml). One of five experiments is shown. Percents are calculated out of total live lymphocytes. (B) ELISA analysis of culture supernatants from splenic B cells stimulated in vitro with LPS or LPS 1 IL-4 (summary of three experiments from Fig. 2A). Open squares are LPS, filled squares are LPS 1 IL-4. (C) ELISA analysis of IgG2b antibody serum levels of mutant and control mice.

Journal: International immunology

Article Title: An expressed neo(r) cassette provides required functions of the 1gamma2b exon for class switching.

doi: 10.1093/intimm/10.11.1683

Figure Lengend Snippet: Fig. 2. In vitro analysis of splenocytes from Iγ2b-targeted chimeric mice. (A) FACS analysis of IgG2b surface expression in mutant and control mice on day 4.5 of splenocytes cultured in RPMI/10% FCS medium containing LPS (20 µg/ml) with or without rIL-4 (20 ng/ml). One of five experiments is shown. Percents are calculated out of total live lymphocytes. (B) ELISA analysis of culture supernatants from splenic B cells stimulated in vitro with LPS or LPS 1 IL-4 (summary of three experiments from Fig. 2A). Open squares are LPS, filled squares are LPS 1 IL-4. (C) ELISA analysis of IgG2b antibody serum levels of mutant and control mice.

Article Snippet: ELISA analysis Splenocyte culture supernatants from day 4.5 or 5 and sera were analyzed for the presence of different Ig isotypes by sandwich ELISA assay as previously described (13,28) using goat anti-mouse IgG2b coating (Southern Biotechnology Associates) and alkaline phosphatase-labeled anti-mouse IgG2b (R12-3) (PharMingen).

Techniques: In Vitro, Expressing, Mutagenesis, Control, Cell Culture, Enzyme-linked Immunosorbent Assay